The conformations of ABC trasporters in lipid environment
ABC exporters are molecular machines which use the energy of the cell to pump diverse chemicals across membranes. They take part in numerous biological processes, such as cell detoxification, and secretion of cholesterol and bile. We are interested in determining the sequence of conformational changes which lead to the coupling between translocation of chemicals and ATPase.
Interaction of NAPE-PLD with bile and membranes
N-acyl phosphatidylethanolamine-specific phospholipase D (NAPE-PLD) generates
key lipid signaling amides that play essential protective roles in multiple physiological and metabolic pathways. We are interested in the structure and function of the regulatory domain of NAPE-PLD, and its interactions with bile, PP1-γ and the membrane.
'Real life' membrane fusion
Membrane fusion takes place in many biological processes, such as: cell-cell fusion during the formation of muscle tissue, and viral infection. While the structures of many fusogens (the proteins which mediate membrane fusion) are already known. We are interested in how fusogens act cooperatively to fuse membranes, and how they interact with other proteins on the membrane (some of which are anti-fusogenic host proteins).